Journal: Frontiers in immunology

Article Title: β2-Adrenoceptor Deficiency Results in Increased Calcified Cartilage Thickness and Subchondral Bone Remodeling in Murine Experimental Osteoarthritis.

doi: 10.3389/fimmu.2021.801505

Figure Lengend Snippet: FIGURE 3 | Analysis of the region of osteophyte formation in the medial condyles. (A) Representative micro-CT images of the medial condyles with (DMM groups) or without (Sham groups) osteophytes 8 weeks after surgery. The quantification of the medial condyle lengths in WT and Adrb2-/- mice 8 weeks after surgery and representative micro-CT images display the medial condyle. Data are presented as box plots with whiskers. Each black circle represents an individual mouse (n=5 per group). Significant p-values are presented as ***p ≤0.001 for comparisons between groups. (B) Immunohistochemical detection and quantification of a2A-AR (dark brown) in the region of osteophyte formation in WT and Adrb2-/- mice 2 and 8 weeks after DMM or Sham surgery (bar: 100 µm). Nuclei are counterstained with hematoxylin (in dark blue). Data are presented as box plots with whiskers. Each black circle represents an individual mouse (n=3 per group). Significant p-values are presented as *p ≤0.05, ***p ≤0.001 for comparisons between groups. ++p≤0.01 when compared to 2 weeks WT DMM.

Article Snippet: Sections were blocked using 2.5% normal horse serum blocking solution for 20 min at room temperature (Cat.No: S-2012, Vector Laboratories, Burlingame, USA) followed by incubation with primary antibodies over night at 4°C (a2A-AR – 14266-1-AP 1:100, Proteintech, Rosemont, US; b2-AR - 1:100, PA5-14117, Thermo Fisher, Rockford, USA; CatK – 1:200 PA05-109605, Thermo Fisher Scientific, Waltham US; and ALP - 1:200 AF2910, RnDsystems, Minneapolis, USA).

Techniques: Micro-CT, Immunohistochemical staining